How do I execute multiple shell commands with a single python subprocess call?

Use semicolon to chain them if they're independent.

For example, (Python 3)

>>> import subprocess
>>> result = subprocess.run('echo Hello ; echo World', shell=True, stdout=subprocess.PIPE, stderr=subprocess.STDOUT)
>>> result
CompletedProcess(args='echo Hello ; echo World', returncode=0, stdout=b'Hello\nWorld\n')

But technically that's not a pure Python solution, because of shell=True. The arg processing is actually done by shell. (You may think of it as of executing /bin/sh -c "$your_arguments")

If you want a somewhat more pure solution, you'll have to use shell=False and loop over your several commands. As far as I know, there is no way to start multiple subprocesses directly with subprocess module.