Call int() function on every list element?

This is what list comprehensions are for:

numbers = [ int(x) for x in numbers ]

In Python 2.x another approach is to use map:

numbers = map(int, numbers)

Note: in Python 3.x map returns a map object which you can convert to a list if you want:

numbers = list(map(int, numbers))

just a point,

numbers = [int(x) for x in numbers]

the list comprehension is more natural, while

numbers = map(int, numbers)

is faster.

Probably this will not matter in most cases

Useful read: LP vs map

If you are intending on passing those integers to a function or method, consider this example:

sum(int(x) for x in numbers)

This construction is intentionally remarkably similar to list comprehensions mentioned by adamk. Without the square brackets, it's called a generator expression, and is a very memory-efficient way of passing a list of arguments to a method. A good discussion is available here: Generator Expressions vs. List Comprehension